Cord Blood and Cord Specimen Collection Guide


This information has been provided for the Doctor, Midwife and Healthcare Professional who has been asked to collect the Umbilical Cord Blood and cord samples on behalf of our newly expectant client at this very special time in their lives.

Cells Limited is the leading provider for the safe storage of stem cells. We provide storage of cord tissues and stem cells extracted from the umbilical cord blood of the newborn.

The umbilical cord or placenta is a rich source of stem cells. These cells can help cure many life threatening diseases. The main use so far has been for blood disorders but potentially these cells could be used to treat illnesses such diabetes, strokes, multiple sclerosis, brain and muscle diseases, Alzheimer's Parkinson's disease and heart muscle cells.

The Doctor, Midwife, Healthcare professional collecting the umbilical cord blood and cord samples plays an important role at this crucial time as these samples can only be collected at the time of birth.

In this leaflet you will find a step by step guide on how to collect the umbilical cord blood and cord samples. The procedure is very simple and quick. It takes no more than few and is completely painless and bares no harm to the child or the mother as it takes place following the safe delivery of the baby and only after the umbilical cord has been cut. The mother/birthing partner sign a disclaimer so that the Doctor, Midwife, Healthcare Professional performing the collection of cord blood and cord samples will not be held liable if due to unforeseen circumstances the collection of the sample (s) was not possible or the amount of cord blood taken proves to be insufficient.

Finally, it is hoped that the cord blood will never have to be used, but in the unlikely event of this happening the family can rest assured with the knowledge that they have the stem cells safely stored and available for immediate use.


Collection must only take place AFTER the cord has been clamped and cut and the Mother and Baby are confirmed safe by the Doctor/Midwife.

Thank you for agreeing to collect the umbilical cord blood. This simple procedure can be performed IN-UTERO or EX-UTERO and normally takes no more than a few minutes. The same method is used for either. It is preferable, if safe to do so, that this procedure is performed IN-UTERO as it has the added advantage of the placenta still pulsating which can help pump blood through the cord into the collection bag. If the cord blood is to be collected EX-UTERO it should be performed as soon as it is safely possible once the placenta has been delivered before the natural clotting process begins. (This needs to be taken into consideration when a physiological delivery of the placenta takes place as the natural clotting process may reduce the amount of cord blood available for collection.) Every effort should be made to collect as much cord blood as possible for the successful storage of stem cells.

The procedure for a caesarean section is the same as it would be for a natural birth EX-UTERO.

Collection of Cord Specimen is performed after collecting the cord blood specimen.

The Healthcare professional collecting the sample must be guided by the Doctor/Midwife attending the delivery. Consideration must also be given to the mother's wishes.


Our CryoCord+ kit is used for the collection of Cord Blood and Cord specimens:

•1 Single blood-pack unit with needle protector:

• 1.6mm (16GA) needle
• Each unit consists of a primary pack containing 35ml CPDA-1 anticoagulant solution for collection of 250ml of blood.

•1 Sterile blister containing:

• Sterile gauze swabs 7.5x7.5cm
• Cleansing wipes
• One Pair of scissors
• One Pincette and one Operation field

1 Sterile tube for cord fragment
1 Absorbing cloth (obligation for transportation)
1 95 kPa plastic bag with hermetic quick lock
2 Gel packs for temperature control during transportation
1 Polystyrene box for transportation

Following documents/items are also provided with the kit separately to facilitate procurement process

1 DHL Courier Clear Flyer/bag
1 Client Identification Form (T be sent with samples)
1 Procurement Form (To be signed by the person who performs the collection and given back to the parents for sending back to Cells Limited offices in Harrow, UK)


This simple procedure must be done in a sterile environment as soon as possible after the safe delivery of the baby and after the cord has been clamped and cut as close to the baby's abdomen as possible.


Collecting the Umbilical Cord Blood Specimen: IN-UTERO


• Take the sterile blood bag out of its packaging and place nearby.

• Clean the umbilical cord with the sterile gauze.

• Disinfect the areas where the needle will be inserted. (a sterile environment is a must)

Collecting the Umbilical Cord Blood Specimen: EX-UTERO


• Prepare the work surface where the cord blood collection will be performed.

• Take the sterile blood bag out of its packaging.

• Place the placenta on a sterile cloth and allow the umbilical cord to hang down over the side. (Gravity can play an important part in this procedure)

• Clean the umbilical cord with the sterile gauze.

• Disinfect the areas where the needle will be inserted. (a sterile environment is a must)


• The needle is covered with a blue cap, remove it.

• Starting at the end of the clamped cord insert the needle attached to the blood bag into the disinfected blood vessel.

• Allow the cord blood to flow into the collection bag to collect as much blood as possible; this may take a few minutes.

• If the cord blood does not flow, very gently turn the needle (the bevel of the needle may be resting on the vessel wall) this should allow the blood to flow.

• If the cord blood still fails to flow, remove the needle from the vessel and insert into the next disinfected vessel further up the cord.

• Gently agitate the collection bag during the procedure in order to mix the cord blood with the CPD anticoagulant.

• It may be necessary to draw blood from several places in the umbilical cord. Please ensure that these areas have been properly sterilised beforehand


• Once the collection bag is full and the blood flow has stopped, remove the needle from the umbilical cord.

• Strip the blood through the tube into the blood bag with the attached white clamp. Securely close the white clamp approx 10cm (4 inches) from the bag.

• Cut off the needle and safely discard into a sharps box in accordance with hospital waste disposal regulations.

• Tie at least two security knots in the collection tube to prevent leakage during transportation (do not use sutures for this)


Cord Specimen collection is performed after the collection of Cord Blood Specimen (Ex-Utero).

Appropriately marked Cord Shipping Tube is provided With the CryoCord+ Kit and contains 25ml sterile saline with a screw-tight lid.

Collecting the Umbilical Cord Specimen: Ex-Utero


• Take this Cord Shipping Tube from the box for collection of a cord fragment for Cryopreservation.

• Select a part of cord away from the punctured site ensuring; the selected part of cord should be as straight as possible and free of blood both internally and externally as far as possible.

• Cut out a 15cm long fragment of cord; the required length is marked at the end of the last page for reference.

• Squeeze the cord fragment gently to remove as much blood as possible and clean it with the alcohol wipes provided.


• Open the Cord Shipping Tube carefully without spilling of liquid already present inside the tube.

• Ensure that the cord fragment is immersed in the liquid present in the Cord Shipping Tube.

• Close the lid of the Cord Shipping Tube tightly but gently ensuring that it is not cross-threaded.


• Pack the Cord Shipping Tube along with the Cord Blood Bag in the Plastic Bag provided with Hermetic Quick Lock for safe transportation.


• Place the cord blood specimen bag and the cord tube onto the absorbent cloth and put them into the small safety bag (pathoseal). Close this safety bag with the self-adhesive strip.

• Put the safety bag (pathoseal) on top of the bottom Gelpack (placed in the White Polystyrene Box).

• Put the second Gelpack on top of the safety bag (pathoseal)

• Close with the isolating lid.

• Enclose the completed Identification Form.

• Close the White Polystyrene box.

• Put the cardboard box in the plastic DHL courier bag and leave it open.

• Finally, complete the supplied Airway Bill (only pick up address, name and signature, other sections will be completed by the courier).

• Give the package containing the Cord Blood and Cord samples and completed Identification Form back to the parents who will then contact the courier for collection and transportation.

The ready pack containing umbilical cord blood and cord samples for transportation should be kept at the room temperature while waiting for the courier to arrive.


• In the event of twins, the entire process for collection of cord blood and cord specimens must be repeated using a separate collection kit for each child. (Each kit will be labelled twin 1 and twin 2)

• It is critical to clearly identify the baby source of each cord collection bag.